The Impact of Barrel Stirring on Cabernet Franc (2017)

Kirsty Harmon

Blenheim Vineyards

Summary

This study examines the effect of barrel stirring during aging on Cabernet Franc.  Cabernet Franc wine (free run and pressings) was settled for 48 hours and then racked into two identical neutral barrels.  After malolactic fermentation, barrels were sulfited and one barrel was left unstirred whereas the other barrel was stirred once per week for three months.  All other treatments between wines were equal.  Wine chemistry was not different between treatments.  The barrel stirred wine had slightly higher levels of acetic acid bacteria, some lactobacillus species, and perhaps Saccharomyces cerevisiae.  O. oeni was higher in the barrel stirred treatment as well.  Tannin may have been slightly lowered by barrel stirring, and catechin may have slightly increased.  Overall, the wines were not found to be significantly different.  Of the people who correctly distinguished the wines, there may have been a slight preference for the unstirred wine (although the tendency to have no preference was also strong).  This study should be repeated again in the future, perhaps with differing levels of lees in each treatment as well and with the turbidity measured. 

Introduction

Marchal et al. (2011) provide an excellent brief review of yeast autolysis in their introduction. Lees are mainly composed of yeast, bacteria, tartaric acid, polysaccharides, and protein-tannin complexes (Zoecklein 2013). Heavy lees generally refers to lees which precipitate 24 hours after fermentation (generally grape particles and large complexes of other lees particulates), and can often lead to offaromas in wine. Light lees precipitate later and are generally beneficial to wine quality, and have less grape particulates and less heavily complexed yeasts and other lees particulates (Zoecklein 2005; Zoecklein 2013). Lees aging can decrease vanilla flavors from oak, and increase toasted flavors (Chatonnet et al. 1992; Tominaga et al. 2000). Others have observed that lees stirring increases yeast character in the wine, decreases fruit and oak character. In some cases, this reduction in oak character can increase the perception of fruit (relative to very oaky control wines) (Zoecklein 2005). 

Lees aging also increases the polysaccharide content of wines, particularly mannoproteins, which may enhance wine protein and tartrate stability (Llaubères et al. 1987; Ledoux et al. 1992; Moine-Ledoux et al. 1997; Feuillat 2003; Zoecklein 2005; Zoecklein 2013). Sur lies aging releases mannoproteins and other cell wall polysaccharides which can enhance the colloidal structure, stability, and aromatic quality of red wines while reducing their astringency, making sur lie aging of red wines important (Zoecklein 2005). Although yeast-derived proteins can increase during lees aging, these proteins are not involved in protein instability (Zoecklein 1991). 

Lees may also act to preserve fruity and varietal characteristics by preventing oxidation and producing a reducing environment (Marchal et al. 2011; Zoecklein 2013). The release of thiols into the wine from yeast has been attributed to lowering reductive characteristics by being able to oxidize methanethiol and ethanethiol into their nonvolatile disulfide forms (Lavigne and Dubourdieu 1996); however, this greatly depends on other factors in the aging process, and could impart a more reductive character to the wine. Yeast glycoproteins from autolysis may also decrease astringency in wines through interaction with phenolic compounds (Escot et al. 2001). Lees autolysis can also impart sweetness to wine (Zoecklein 2005; Marchal et al. 2001), which may be in part due to sweet peptide fractions released during cell autolysis. One such fraction appears to be derived from heat shock proteins (Hsp12p) (Marchal et al. 2011), which is expressed from high temperature, ethanol, oxidative stress, and glycerol concentrations (Varela et al. 1995). All of these factors are present under winemaking conditions (Marchal et al. 2011). The breakdown of peptides can result in aromatic precursors in wines (Zoecklein 2005), but may also provide more nitrogen for spoilage organisms to consume. Many of these impacts of lees aging can be affected by winemaking practices, such as frequency of stirring, amount of lees present, amount of oxygen ingress, pectinase/glucosidase enzyme additions (such as Extralyse by Laffort), and perhaps even quality of lees. This study examines the impact of one such lees stirring regime on the chemical and sensory qualities of red wine.

Results and Discussion

Wine chemistry was not different between treatments.  The barrel stirred wine had slightly higher levels of acetic acid bacteria, some lactobacillus species, and perhaps Saccharomyces cerevisiae.  O. oeni was higher in the barrel stirred treatment as well.  Tannin may have been slightly lowered by barrel stirring, and catechin may have slightly increased.



Wine Chemistry

 

Ethanol (%vol/vol)

Residual Sugar (g/L)

pH

TA (g/L)

Volatile Acidity (g/L)

Malic Acid (g/L)

Lactic Acid (g/L)

Ammonia (mg/L)

NOPA (mg N/L)

YAN (mg N/L)

Total SO2 (ppm)

Free SO2 (ppm)

Molecular SO2 (ppm)

No Stirring

13.76

<1

3.71

4.04

0.51

<0.15

1.41

<10

32

36

84

40

0.76

Stirring

13.75

<1

3.70

4.03

0.49

<0.15

1.44

<10

31

35

82

41

0.79

% Change

0%

 

0%

0%

-4%

 

2%

 

-3%

-3%

-2%

3%

4%

Results from ICV in Mid April, Except Nitrogen from ETS
 



Wine Microbiology

 

Acetic Acid Bacteria (cells/mL)

L. brevis, hilgardii, and fermentum (cells/mL)

L. plantarum, casei, and mali (cells/mL)

L. kunkeei (cells/mL)

O. oeni (cells/mL)

Pediococcus sp. (cells/mL)

B. bruxellensis (cells/mL)

S. cerevisiae (cells/mL)

Z. bailii (cells/mL)

No Stirring

127000

<10

120

<10

3400000

2610

<10

11500

<10

Stirring

260000

<10

430

<10

>10000000

2300

<10

19400

<10

% Change

105%

 

258%

   

-12%

 

69%

 

Results from ETS in Mid April
 



Color Profile

 

A420

A520

A620

Hue (420/520)

Intensity (420 + 520 + 620)

No Stirring

0.223

0.256

0.072

0.871

0.551

Stirring

0.228

0.259

0.076

0.880

0.563

% Change

2%

1%

6%

1%

2%

Results from ICV in Mid April
 



Phenolic Profile

 

Caffeic Acid (mg/L)

Caftaric Acid (mg/L)

Catechin (mg/L)

Epicatechin (mg/L)

Gallic Acid (mg/L)

No Stirring

8

25

24

20

40

Stirring

8

25

31

24

40

% Change

0%

0%

29%

20%

0%

Results from ETS in Mid April
 



Phenolic Profile

 

Malvidin glucoside (mg/L)

Monomeric Anthocyanins (mg/L)

Polymeric Anthocyanins (mg/L)

Quercetin (mg/L)

Quercetin Glycosides (mg/L)

Tannin (mg/L)

Total Anthocyanins (mg/L)

Resveratrol (cis and trans) (mg/L)

No Stirring

167

279

15

8

16

304

294

0.2

Stirring

174

290

16

8

16

266

306

0.2

% Change

4%

4%

7%

0%

0%

-13%

4%

0%

Results from ETS in Mid April
 


For the triangle test on May 16, of 21 people who answered, 9 people chose the correct wine (43%), suggesting that these wines were not significantly different.  In general, of those who answered correctly, 3 preferred the no stirring wine, 1 preferred the stirred wine, and 5 had no preference.  For the descriptive analysis, there were no strong trends for the descriptors used in this study.  There was a slight tendency for the wine that was not stirred to have lower Bitterness.  Judges generally felt that these wines were very green, which seemed to override other differences.

 

 

For the triangle test on May 30, of 28 people who answered, 12 people chose the correct wine (43%), suggesting that the wines were not significantly different.  In general, of those who correctly guessed the wines, 5 preferred no stirring, 3 preferred stirring, and 4 had no preference.  For the descriptive analysis on May 30, there were no strong trends for the descriptors used in this study.  The sensory data was mixed, and minor trends are hard to determine.

 

 

Overall, the wines were not found to be significantly different.  Of the people who correctly distinguished the wines, there may have been a slight preference for the unstirred wine (although the tendency to have no preference was also strong).  This study should be repeated again in the future, perhaps with differing levels of lees in each treatment as well and with the turbidity measured.

 

Methods

Cabernet Franc wine (blend of free run and pressings) was settled for 48 hours. Wine was racked to identical neutral barrels. ML Silver malolactic bacteria was added to each barrel at a rate of 2g/barrel. Once malolactic fermentation was complete (enzymatic assay) wines were sulfited at a rate of 75 ppm.   One barrel was left unstirred, and another barrel was stirred roughly once per week 3 months.  All other treatments between wines were equal.

These wines were tasted on May 16 and May 30.  For the triangle test, descriptive analysis, and preference analysis for the May 16 tasting, anybody who did not answer the form were removed from consideration for both triangle, degree of difference, and preference.  Additionally, anybody who answered the triangle test incorrectly were removed from consideration for degree of difference and preference.  Additionally, any data points for preference which did not make sense (such as a person ranking a wine and its replicate at most and least preferred, when they correctly guessed the odd wine) were removed.  

In order to balance the data set to perform statistical analysis for descriptive analysis on the May 16 tasting, any judge who had not fully completed the descriptive analysis ratings were removed.  In order to then make the number of judges between groups equivalent, one judge from group 1 was transferred to group 3.  This resulted in a final data set of 3 groups, each with 6 judges (considered as replications within groups, and groups were considered as assessors).  Data was analyzed using Panel Check V1.4.2.  Because this is not a truly statistical set-up, any results which are found to be statistically significant (p<0.05) will be denoted as a “strong trend” or a “strong tendency,” as opposed to general trends or tendencies.  The statistical significance here will ignore any other significant effects or interactions which may confound the results (such as a statistically significant interaction of Judge x Wine confounding a significant result from Wine alone).  The descriptors used in this study were Fruit Intensity, Herbaceous/Green, Overall Aromatic Intensity, Bitterness, Astringency, and Body.

The same procedures for data analysis were used on the May 30 tasting.  For the descriptive analysis in this tasting, one judge was transferred from group 3 to both groups 1 and 2, so that each group had 9 judges, for a total of 27 judges.


References

Chatonnet, P., Dubourdieu, D., and Boidron, J. N. 1992. Incidence des conditions de fermentation et d’èlevage des vins blancs secs en barriques sur leur composition en substances cèdèes par le bois de chêne. Sci. Aliments. 12:665–685. 
Escot, S., Feuillat, M., Dulau, L., and Charpentier, C. 2001. Release of polysaccharides by yeasts and the influence of released polysaccharides on colour stability and wine astringency. Aust. J. Grape Wine Res. 7:153–159.
Feuillat, M. 2003. Yeast macromolecules: Origin, composition, and enological interest. Am. J. Enol. Vitic. 54:211- 213. Lavigne, V. and Dubourdieu, D. 1996. Demonstration and interpretation of the yeast lees ability to adsorb certain volatile thiols contained in wine. J. Int. Sci. Vigne Vin. 30:201–206. 
Ledoux, V., Dulau, L., and Dubourdieu, D. 1992. Interprètation de l’amèlioration de la stabilitè protèique des vins au cours de l’èlevage sur lies. J. Int. Sci. Vigne Vin. 26:239–251 
Llaubères, R.-M., Dubourdieu, D., and Villettaz, J.-C. 1987. Exocellular polysaccharides from Saccharomyces in Wine. J. Sci. Food Agric. 41:277–286. 
Marchal, A., Marullo, P., Moine, V., and Dubourdieu, D. 2011. Influence of yeast macromolecules on sweetness in dry wines: role of the Saccharomyces cerevisiae protein Hsp12. Journal of Agricultural and Food Chemistry. 59:2004-2010. 
Moine-Ledoux, V., Perrin, A., Paladin, I., and Dubourdieu, D. 1997. Premiers rèsultats de stabilisation tartrique des vins par addition de mannoprotèines purifièes (Mannostab). J. Int. Sci. Vigne Vin. 31:23–31. 
Tominaga, T., Blanchard, L., Darriet, P., and Dubourdieu, D. 2000. A powerful aromatic volatile thiol, 2- furanmethanethiol, exhibiting roast coffee aroma in wines made from several Vitis vinifera grape varieties. J. Agric. Food Chem. 48:1799–1802. 
Varela, J. C. S., Praekelt, U. M., Meacock, P. A., Planta, R. J., and Mager, W. H. 1995. The Saccharomyces cerevisiae HSP12 gene is activated by the high- osmolarity glycerol pathway and negatively regulated by protein kinase A. Mol. Cell. Biol. 15:6232–6245.
Zoecklein, B. 1991. Protein stability determination in juice and wine. Virginia Cooperative Extension. http://www.apps.fst.vt.edu/extension/enology/downloads/ProteinS.pdf.
Zoecklein, B. 2013. Nature of wine lees. Practical Winery and Vineyard. July. http://www.apps.fst.vt.edu/extension/enology/downloads/wm_issues/Nature%20of%20Wine%20 Lees.pdf
Zoecklin, B. 2005. Lees Management. Enology Notes #106. http://www.apps.fst.vt.edu/extension/enology/EN/106.html.

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